RPR Test For Syphilis - test rapid plasma reagin and its automated modifications - automatic reagin test (ART, Automated Reagin Test). These tests can be read visually by the presence of colored substances in the prepared antigen. RPR test was developed for field conditions and is now the most common non-treponemal test. He carried on plastic pancetta with imprinted circles. In formulating a quantitative or qualitative modifications of the standard reaction of undiluted serum or serial dilutions are mixed with the antigen content of coal particles. Tablet with the antigen and serum spun at 1000 rpm for 8 min. Antigen is not attached to these particles, but in the presence of serum anti-cardio lipping antibody flocculation occurs antigen-antibody complexes, particles of coal as would fall into the trap in the formed network, and coagulate. The test results are read in a damp place under bright test immediately after the reaction. The test is used unheated serum.

TRUST (Toluidin Red Unheated Serum Test) - test with toluidine red unheated serum and. In this test, the standard particle toluidine red azote dye is added to the stabilized core antigen, and visualization of expressed better than by adding carbon particles in the RPR. Currently TRUST test is not widely used.

VDRL (under the name of the laboratory-developer - Venereal Disease Research Laboratory). In this test, freshly prepared antigen and inactivated serum are mixed in a glass-hole mechanical rotation. If the serum contains antibodies to cardio lipping antigen, formed aggregates in the form of short rod-shaped structures, observed microscopically. Used quantitative modification VDRL serum dilutions up to 1:64. VDRL is the only test recommended for the study of cerebrospinal fluid.

USR (Unheated Serum Reagins) - test definition of active plasma reagin. Antigen in this test a bit more expensive antigen VDRL, however, it is stabilized by adding choline chloride and EDTA and no need for daily cooking.

For non-treponemal tests is also a method of indirect ELISA with cardio lipping antigen. In domestic practice as qualifying non-treponemal tests are used micro reaction with plasma and inactivated serum (MP) and complement fixation with cardio lipping antigen (RCC).

Characteristic of all non-treponemal tests currently in use, like. All of them are relatively labor-intensive and simple in execution. The sensitivity of all tests is approximately the like when setting VDRL (about 70% - with primary syphilis and 100% - with secondary). Non-treponemal tests are less sensitive in the primary and latent syphilis. Antibodies are defined by non-treponemal tests usually appear within 1 - 4 weeks after the chancre formation (about 1-1, 5 months after infection). Most patients with non-treponemal reactions become almost negative in the first year after treatment for primary syphilis.